s100b antibody (S100 Calcium Binding Protein B) (pSer100)

Details for Product anti-S100B Antibody No. ABIN3021049
Antigen
  • NEF
  • S100
  • S100-B
  • S100beta
  • S100P
  • AI850290
  • Bpb
  • si:dkey-110p14.2
  • wu:fq18b10
  • zgc:92776
  • S100 calcium binding protein B
  • S100 protein, beta polypeptide, neural
  • S100 calcium binding protein, beta (neural)
  • S100B
  • S100b
  • s100b
Epitope
pSer100
42
15
13
9
9
7
6
6
4
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
373
247
219
171
40
25
8
7
4
2
2
1
1
1
Host
Rabbit
211
202
11
4
3
1
Clonality
Polyclonal
Conjugate
This s100b antibody is un-conjugated
38
27
14
9
9
9
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
344
187
163
159
114
63
59
28
25
22
16
14
9
8
3
3
2
2
1
1
1
1
Options
Immunogen Recombinant Protein of human pSer100 B
Isotype IgG
Purification Affinity purification
Plasmids, Primers & others Plasmids, Primers & others s100b products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name S100B (S100B Antibody Abstract)
Background Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development.Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities. S100B is abundantly expressed in astrocytes and is commonly used as an astrocytic marker in studies of the mammalian CNS. S100B is also expressed in immature and mature myelinating oligodendrocytes that are chondroitin sulfate proteoglycan (NG2)-positive.
Synonyms: S100B, NEF, S100, S100beta
Gene ID 6285
UniProt P04271
Pathways Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C
Supplier Images
Image no. 1 for anti-S100 Calcium Binding Protein B (S100B) (pSer100) antibody (ABIN3021049) Western blot analysis of extracts of various cell lines, using S100B antibody.
Product cited in: Yao, Zhao, Tang, Xiong, Zhao, Liu, Dong, Zou, Cai: "Blockade of β-catenin signaling attenuates toluene diisocyanate-induced experimental asthma." in: Allergy, Vol. 72, Issue 4, pp. 579-589, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Yao, Zhao, Tang, Liang, Liu, Dong, Zou, Cai: "The receptor for advanced glycation end products is required for β-catenin stabilization in a chemical-induced asthma model." in: British journal of pharmacology, Vol. 173, Issue 17, pp. 2600-13, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

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