Minichromosome Maintenance Complex Component 2 (MCM2) antibody

Details for Product No. ABIN3021315
Antigen
  • CG7538
  • DmMCM2
  • DmMcm2
  • DmeMCM2
  • Dmel\\CG7538
  • MCM2
  • MCM2-7
  • MCM2_DROME
  • McM2
  • PCR3
  • dMCM2
  • l(3)rL074
  • XMcm2
  • bm28
  • ccnl1
  • cdc19
  • cdcl1
  • mitotin
  • cb737
  • chunp6905
  • BM28
  • CCNL1
  • CDCL1
  • D3S3194
  • MITOTIN
  • MCM7
  • AA959861
  • AW476101
  • Mcmd2
  • mKIAA0030
  • Minichromosome maintenance 2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance complex component 2 L homeolog
  • Mcm2
  • mcm2
  • MCM2
  • mcm2.L
Reactivity
Mouse (Murine), Rat (Rattus), Human
214
104
63
11
11
10
9
7
5
3
3
2
2
1
1
Host
Rabbit
174
61
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
8
8
4
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
199
110
91
57
35
18
17
16
13
6
4
4
4
3
2
Options
Immunogen Recombinant protein of human MCM2
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name MCM2 (MCM2 Antibody Abstract)
Background The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis. MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3, 9).
Synonyms: MCM2, BM28, CCNL1, CDCL1, D3S3194, KIAA0030, MGC10606, MITOTIN, cdc19
Gene ID 4171
UniProt P49736
Pathways DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C
Supplier Images
Image no. 1 for anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibody (ABIN3021315) Western blot analysis of extracts of various cell lines, using MCM2 antibody.
Image no. 2 for anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibody (ABIN3021315) Immunohistochemistry of paraffin-embedded human colon using MCM2 antibody.
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