SAFB2 antibody (AA 345-357)

Catalog No. ABIN302304

Product Details anti-SAFB2 Antibody

Target: SAFB2 (Scaffold Attachment Factor B2 (SAFB2))

Binding Specificity: AA 345-357

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAFB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Scaffold Attachment Factor B (SAFB).

Characteristics: Synonyms: Scaffold attachment factor B2, SAF-B2, KIAA0138

Purification: Protein G column

Immunogen: A synthetic peptide corresponding to amino acids 345-357 of the human HET proteinAA Sequence: PEARDSKEDGRKF

Clone: 6F7

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Immunoflourescence. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 - -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SAFB2

Target: SAFB2 (Scaffold Attachment Factor B2 (SAFB2))

Alternative Name: SAFB2 (SAFB2 Products)

Background: Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.Synonyms: KIAA0138, SAF-B2, Scaffold attachment factor B2

Gene ID: 9667

UniProt: Q14151