FOXP3 antibody (N-Term)
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- Target See all FOXP3 Antibodies
- FOXP3 (Forkhead Box P3 (FOXP3))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FOXP3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.
- Clone
- FHBP3-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product FOXP3 Primary Antibody
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- Application Notes
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Titering of the FOXP3 antibody may be required for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- FOXP3 (Forkhead Box P3 (FOXP3))
- Alternative Name
- FOXP3 (FOXP3 Products)
- Background
- The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
- Gene ID
- 50943
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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