Helicobacter Pylori antibody
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- Target See all Helicobacter Pylori (H. pylori) products
- Helicobacter Pylori (H. pylori)
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Reactivity
- Helicobacter pylori
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Helicobacter Pylori antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Purification
- Protein A affinity chromatography
- Immunogen
- Total sonicate of Helicobacter ylori was used as the immunogen for this antibody.
- Isotype
- IgG
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1mg/ml in 1X PBS with 0.05% sodium azide; BSA-free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Helicobacter Pylori antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Helicobacter Pylori (H. pylori)
- Alternative Name
- Helicobacter Pylori (H. pylori Products)
- Target Type
- Virus
- Background
- The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
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