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MYL9 antibody (AA 12-27)

MYL9 Reactivity: Human, Mouse, Rat, Avian, Mammalian EIA, IHC (p), IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN303105
$739.20
Plus shipping costs $45.00
50 μg
Shipping to: United States
Available
  • Target See all MYL9 Antibodies
    MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
    Binding Specificity
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 12-27
    Reactivity
    • 17
    • 9
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Avian, Mammalian
    Host
    • 16
    • 1
    Rabbit
    Clonality
    • 16
    • 1
    Polyclonal
    Conjugate
    • 13
    • 2
    • 1
    • 1
    This MYL9 antibody is un-conjugated
    Application
    • 12
    • 9
    • 9
    • 5
    • 2
    • 2
    • 1
    • 1
    Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    Amino acids 12-27 of Human Myosin light chain protein.
    Cross-Reactivity (Details)
    Species reactivity (expected):Avian, Mammal, Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Immunoaffinity Chromatography.
    Immunogen
    Synthetic peptide - KLH conjugated.
  • Application Notes
    ELISA (1/5000-1/20000). Western Blot (1/500-1/2000). Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by Alkalinephosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.71 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
    Alternative Name
    MYL9 (MYL9 Products)
    Synonyms
    LC20 antibody, MLC-2C antibody, MLC2 antibody, MRLC1 antibody, MYRL2 antibody, AI327049 antibody, MLC20 antibody, Mylc2c antibody, RLC-C antibody, myosin antibody, regulatory antibody, MRCL3 antibody, MRLC2 antibody, MYL9 antibody, zgc:103467 antibody, myosin light chain 9 antibody, myosin, light polypeptide 9, regulatory antibody, myosin light chain 9 L homeolog antibody, myosin, light chain 9, regulatory antibody, myosin, light chain 12A, regulatory, non-sarcomeric antibody, myosin, light chain 9a, regulatory antibody, MYL9 antibody, Myl9 antibody, myl9.L antibody, MYL12A antibody, myl9a antibody
    Background
    Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.Synonyms: LC20, MLC-2C, MLC2, MRLC1, MYRL2, Myosin RLC, Myosin regulatory light chain 2, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, smooth muscle
    Gene ID
    10398
    NCBI Accession
    NP_006088
    UniProt
    P24844
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