HINT1 antibody (Middle Region)

Catalog No. ABIN3042445

Product Details anti-HINT1 Antibody

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Binding Specificity: AA 59-92, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HINT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB,IHC-P in Human,Mouse,Rat.

Sequence: HISQISVAED DDESLLGHLM IVGKKCAADL GLNK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB,IHC-P in Human,Mouse,Rat.
Gene Name: histidine triad nucleotide binding protein 1
Protein Name: Histidine triad nucleotide-binding protein 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK), different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HINT1

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Alternative Name: HINT1 (HINT1 Products)

Background: Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/β-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.

Synonyms: HINT 1 antibody|HINT1 antibody|HINT1_HUMAN antibody|Histidine triad nucleotide binding protein 1 antibody|Histidine triad nucleotide-binding protein 1 antibody|PKCI 1 antibody|PKCI-1 antibody|PKCI1 antibody|PRKCNH1 antibody|Protein kinase C inhibitor 1 antibody| Protein kinase C interacting protein 1 antibody|Protein kinase C-interacting protein 1 antibody

Gene ID: 3094

UniProt: P49773