ALDH1A1 antibody (Middle Region)

Catalog No. ABIN3044255

Product Details anti-ALDH1A1 Antibody

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Binding Specificity: AA 283-303, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Retinal dehydrogenase 1(ALDH1A1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: DLDNAVEFAH HGVFYHQGQC C

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Retinal dehydrogenase 1(ALDH1A1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: aldehyde dehydrogenase 1 family, member A1
Protein Name: Retinal dehydrogenase 1(RALDH 1/RalDH1)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ALDH1A1(283-303aa DLDNAVEFAHHGVFYHQGQCC), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-ALDH1A1 antibody (ABIN3044255)

Shao, Fan, Ma, Wu: "Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 4991-4998, (2017) (PubMed).

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1 (ALDH1A1 Products)

Background: ALDH1A1(Aldehyde dehydrogenase 1 family, member A1), also called ALDH1, RALDH1 or ALDH, LIVER CYTOSOLIC, is an enzyme that in humans is encoded by the ALDH1A1 gene. And this protein belongs to the aldehyde dehydrogenases family of proteins. The ALDH1A1 gene is mapped on 9q21.13. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea.ALDH1A1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. The ALDH1 gene is about 53 kb long and is divided into 13 exons. Retinaldehyde is generated by ADH1 from retinol, and its concentration is determined in large part by its subsequent catabolism by RALDH1 to retinoic acid.

Synonyms: Acetaldehyde dehydrogenase 1 antibody|AHD2 antibody|AL1A1_HUMAN antibody|ALDC antibody|Aldehyde dehydrogenase 1 family member A1 antibody|Aldehyde dehydrogenase 1 soluble antibody|Aldehyde dehydrogenase 1A1 antibody|Aldehyde Dehydrogenase antibody|Aldehyde dehydrogenase cytosolic antibody|Aldehyde dehydrogenase family 1 member A1 antibody|Aldehyde dehydrogenase liver cytosolic antibody|ALDH 1 antibody|ALDH 1A1 antibody|ALDH class 1 antibody|ALDH-E1 antibody|ALDH1 A1 antibody|ALDH1 antibody|ALDH11 antibody|ALDH1A1 antibody|ALHDII antibody|cytosolic antibody|MGC2318 antibody|PUMB1 antibody|RALDH 1 antibody|RalDH1 antibody|Retinal dehydrogenase 1 antibody

UniProt: P00352

Pathways: Dopaminergic Neurogenesis