Myogenin antibody (AA 30-224)

Catalog No. ABIN306849

Product Details anti-Myogenin Antibody

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Binding Specificity: AA 30-224

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Myogenin, Myogenic factor 4, Myf-4,Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.

Clone: F12B

Isotype: IgG1

Application Details

Application Notes: This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide,X1415M, clone F12B recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin (MYOG Products)

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development