ABCB5 antibody (Internal Region)

Catalog No. ABIN349592

Supplier Product No.: 600-401-a77

Product Details anti-ABCB5 Antibody

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-a77

Supplier: Rockland

Purpose: ABCB5 Antibody

Cross-Reactivity (Details): This antibody is specific for human ABCB5 protein.

Characteristics: Synonyms: rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200

Application Note: This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting.  Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:20,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.29 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ABCB5 (ABCB5 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.

Gene ID: 340273, 56849536

UniProt: Q2M3G0