GABRG2 antibody (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2) (pSer327)

Details for Product anti-GABRG2 Antibody No. ABIN361470
Target
Binding Specificity
pSer327
15
13
8
7
7
7
4
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat
69
53
52
13
11
6
6
6
5
5
3
3
3
2
Host
Rabbit
67
9
1
Clonality
Polyclonal
Conjugate
This GABRG2 antibody is un-conjugated
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
59
31
21
14
6
4
2
2
1
1
1
Options
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH
Specificity Specific for ~45k GABAA receptor gamma2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by lambda phosphatase treatment.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Predicted Reactivity bovine, canine, chicken, human, non-human primate
Purification Antigen Affinity Purified from Pooled Serum
Target
Alternative Name GABRG2 (GABRG2 Antibody Abstract)
Background Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. It has recently been suggested that PKC regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)
Molecular Weight '45 kDa
Gene ID 29709
UniProt P18508
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Buffer 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage -20 °C
Images
Image no. 1 for anti-gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2) (pSer327) antibody (ABIN361470) Western blots of rat cortex showing specific immunolabeling of the ~45k GABAA gamma2 ...
Product cited in: Bohnsack, Carlson, Morrow: "Differential regulation of synaptic and extrasynaptic α4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons." in: Neuropharmacology, Vol. 105, pp. 124-32, 2016 (PubMed).