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HSP27 antibody (pSer15)

Name: HSP27 antibody (pSer15)
SKU: ABIN362171
Price: 240.9 USD
Availability: InStock

HSPB1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

(1 reference)

Catalog No. ABIN362171

Datasheet as PDF

Target See all HSP27 (HSPB1) Antibodies

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
Binding Specificity
40

33

32

16

16

8

8

6

6

5

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer15
Reactivity
295

99

86

19

13

13

13

10

7

5

4

3

3

2

1

1
Human
Host
234

66
Rabbit
Compatible Secondaries
Clonality
208

91
Polyclonal
Conjugate
133

20

15

11

10

7

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
Application
245

102

99

87

63

47

46

42

41

40

15

12

10

7

7

3

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity

The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen

Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG

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anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
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Application Notes

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." in: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, (2012) (PubMed).
Target

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name

HSP27 (HSPB1 Products)

Background

Involved in stress resistance and actin organization.

Molecular Weight

27 kDa

NCBI Accession

NP_001531

UniProt

P04792

Pathways

MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling