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HSP27 antibody (Heat Shock 27kDa Protein 1) (pSer15) Primary Antibody

HSPB1 Reactivity: Human IF, IHC, WB Host: Rabbit Polyclonal unconjugated
Pubmed (1)
Catalog No. ABIN362172
$306.90
Plus shipping costs $45.00
100 μL ABIN362172
100 μL ABIN362172
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
  • Target
    HSP27 (HSPB1)
    Binding Specificity
    • 41
    • 36
    • 30
    • 16
    • 13
    • 12
    • 10
    • 8
    • 8
    • 8
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer15
    Reactivity
    • 340
    • 138
    • 128
    • 43
    • 41
    • 29
    • 27
    • 22
    • 19
    • 17
    • 14
    • 11
    • 9
    • 7
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 251
    • 95
    • 1
    Rabbit
    Clonality
    • 232
    • 114
    Polyclonal
    Conjugate
    • 162
    • 26
    • 18
    • 14
    • 13
    • 10
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSP27 antibody is un-conjugated
    Application
    • 249
    • 122
    • 112
    • 105
    • 76
    • 60
    • 57
    • 54
    • 40
    • 30
    • 18
    • 16
    • 14
    • 10
    • 9
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." in: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, 2012 (PubMed).

  • Target
    HSP27 (HSPB1)
    Alternative Name
    HSP27 (HSPB1 Antibody Abstract)
    Synonyms
    CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, 27kDa, Hsp27, cb153, cb660, hsp1, hsp25, hsp27, id:ibd2821, sb:cb660, zgc:103437, HSPB1, cmt2f, hs.76067, hsp28, heat shock protein family B (small) member 1, heat shock protein 1, heat shock protein, alpha-crystallin-related, 1, heat shock protein family B (small) member 1 L homeolog, HSPB1, Hspb1, hspb1, hspb1.L
    Background
    Involved in stress resistance and actin organization.
    Molecular Weight
    27 kDa
    NCBI Accession
    NP_001531
    UniProt
    P04792
    Pathways
    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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