HSP90AB1 antibody (pSer254)

Catalog No. ABIN362221

Product Details anti-HSP90AB1 Antibody

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Binding Specificity: pSer254

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AB1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer254 (V-G-S (p) -D-E) derived from Human HSP90B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for HSP90AB1

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Alternative Name: HSP90B (HSP90AB1 Products)

Background: Molecular chaperone. Has ATPase activity.

Molecular Weight: 92 kDa

NCBI Accession: NP_031381

UniProt: P08238

Pathways: Regulation of Cell Size