HSP90AB1 antibody (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1) (pSer254)

Catalog No. ABIN362222

Product Details anti-HSP90AB1 Antibody

Target: HSP90AB1

Binding Specificity: pSer254

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AB1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer254 (V-G-S (p) -D-E) derived from Human HSP90B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for HSP90AB1

Target: HSP90AB1

Alternative Name: HSP90B (HSP90AB1 Antibody Abstract)

Synonyms: D6S182, HSP84, HSP90B, HSPC2, HSPCB, GRP94, TRA1, hsp90b, 90kDa, AL022974, C81438, Hsp84, Hsp84-1, Hsp90, Hspcb, Hsp70, Hsp70-1, Hsp70.1, hsp68, HSP90-BETA, hsp90beta, wu:fa29f01, wu:fa91e11, wu:fd59e11, wu:gcd22h07, HSP90, heat shock protein 90 alpha family class B member 1, heat shock protein 90 beta family member 1, Heat Shock Protein 90, endoplasmic reticulum, heat shock protein 90B, heat shock protein 90 alpha (cytosolic), class B member 1, heat shock protein 1B, heat shock protein 90kDa alpha family class B member 1 S homeolog, heat shock protein 90, alpha (cytosolic), class B member 1, HSP90AB1, HSP90B1, HSP90B, hsp90ab1, Hsp90ab1, Hspa1b, hsp90ab1.S

Background: Molecular chaperone. Has ATPase activity.

Molecular Weight: 92 kDa

NCBI Accession: NP_031381

UniProt: P08238

Pathways: Regulation of Cell Size