ANP32B antibody (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B) (C-Term)

Catalog No. ABIN374313

Product Details anti-ANP32B Antibody

Target: ANP32B

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Binding Specificity: C-Term

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• AA 17-45 10
• C-Term 8
• AA 16-45 7
• N-Term 6
• AA 1-251 3
• AA 1-17 1
• AA 1-50 1
• AA 10-90 1
• AA 36-85 1
• AA 37-86 1

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

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• Human 52
• Mouse 24
• Rat 23
• Pig 3
• Cow 2
• Dog 2
• Guinea Pig 2
• Horse 2
• Sheep 2
• Bat 1
• Monkey 1
• Rabbit 1
• Xenopus laevis 1
• Zebrafish (Danio rerio) 1

Host: Goat

Clonality: Polyclonal

Conjugate: This ANP32B antibody is un-conjugated

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• Un-conjugated 20
• Biotin 4
• FITC 4
• HRP 4
• APC 3
• Alkaline Phosphatase (AP) 3
• PE 3
• Alexa Fluor 350 1
• Alexa Fluor 488 1
• Alexa Fluor 555 1
• Alexa Fluor 594 1
• Alexa Fluor 647 1
• Alexa Fluor 680 1
• Alexa Fluor 750 1
• Cy3 1
• Cy5 1
• Cy5.5 1
• Cy7 1

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

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• Western Blotting (WB) 40
• ELISA 28
• Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13
• Immunofluorescence (IF) 4
• Immunohistochemistry (IHC) 3
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3
• Immunoprecipitation (IP) 1

Sequence: C-KRKRETDDEG EDD

Specificity: This antibody does NOT recognize the TNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006392.1 Genename: ANP32B

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/1000. Western blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human lymph node lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for ANP32B

Target: ANP32B

Alternative Name: ANP32B (ANP32B Antibody Abstract)

Synonyms: APRIL, PHAPI2, SSP29, 2410015B15Rik, PAL31, PHAPI2a, Ssp29, cb908, fa99a11, wu:fa28c10, wu:fa99a11, wu:fb40e12, zgc:77745, hLAMP-1, MGC80871, ANP32B, MGC69373, SLAN, acidic nuclear phosphoprotein 32 family member B, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic nuclear phosphoprotein 32 family member B L homeolog, acidic leucine-rich nuclear phosphoprotein 32 family member B, ANP32B, Anp32b, anp32b, anp32b.L, LOC494442

Background: PHAPI2 / APRIL is a multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. It is required for the progression from the G1 to the S phase. It is an anti-apoptotic protein which functions as a caspase-3 inhibitor. It has no phosphatase 2A (PP2A) inhibitor activity.Synonyms: APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, PHAPI2, SSP29, Silver-stainable protein SSP29

Gene ID: 10541

NCBI Accession: NP_006392

UniProt: Q92688