ANP32B antibody (C-Term)

Catalog No. ABIN374313

Product Details anti-ANP32B Antibody

Target: ANP32B (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ANP32B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-KRKRETDDEG EDD

Specificity: This antibody does NOT recognize the TNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006392.1 Genename: ANP32B

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/1000. Western blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human lymph node lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for ANP32B

Target: ANP32B (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B))

Alternative Name: ANP32B (ANP32B Products)

Background: PHAPI2 / APRIL is a multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. It is required for the progression from the G1 to the S phase. It is an anti-apoptotic protein which functions as a caspase-3 inhibitor. It has no phosphatase 2A (PP2A) inhibitor activity.Synonyms: APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, PHAPI2, SSP29, Silver-stainable protein SSP29

Gene ID: 10541

NCBI Accession: NP_006392

UniProt: Q92688