Mouse anti-Human IgG4 (Fc Region) Antibody (PE)

Details for Product No. ABIN376574
Antigen
Epitope
Fc Region
9
6
3
2
1
1
1
1
Reactivity
Human
41
2
Host
Mouse
33
4
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
PE
5
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA
Options
Immunogen Human IgG4 myeloma protein
Clone HP6025
Isotype IgG1
Specificity Human IgG4 Fc, Mr 146 kDa
Characteristics Mouse Anti-Human IgG4 Fc-PE
Purification Purified
Antigen
Application Notes
  • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Microarray - Reported in literature , Multiplex - Reported in literature , Purification - Reported in literature
  • Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Supplier Images
Image no. 1 for Mouse anti-Human IgG4 (Fc Region) antibody (PE) (ABIN376574) FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobuli...
Product cited in: Violari, Cotton, Kuhn, Schramm, Paximadis, Loubser, Shalekoff, Da Costa Dias, Otwombe, Liberty, McIntyre, Babiker, Gibb, Tiemessen: "A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation." in: Nature communications, Vol. 10, Issue 1, pp. 412, 2019 (PubMed).

Howie, Delaney, Wang, Er, Vidarsson, Stegmann, Kapp, Lebedev, Wu, AuBuchon, Zimring: "Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, 2017 (PubMed).

Song, Franco, Kao, Yu, Huang, Ho: "Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients." in: Journal of virology, 2010 (PubMed).