PKC alpha antibody (C-Term)

Catalog No. ABIN391001

Product Details anti-PKC alpha Antibody

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Binding Specificity: AA 299-329, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Predicted Reactivity: M, Rb, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PKC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-329 amino acids from the C-terminal region of human PKC alpha.

Clone: RB01012

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PKC alpha antibody (ABIN391001)

Jaafar, Zeiller, Pirola, Di Grazia, Naro, Vidal, Lefai, Némoz: "Phospholipase D regulates myogenic differentiation through the activation of both mTORC1 and mTORC2 complexes." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22609-21, (2011) (PubMed).

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PKC alpha (PKCa Products)

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Molecular Weight: 76750

Gene ID: 5578

NCBI Accession: NP_002728

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling