GCN2 antibody (N-Term)

Catalog No. ABIN391197

Product Details anti-GCN2 Antibody

Target: GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This GCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCN2.

Clone: RB8239

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-GCN2 antibody (ABIN391197)

Fougeray, Mami, Bertho, Beaune, Thervet, Pallet: "Tryptophan depletion and the kinase GCN2 mediate IFN-?-induced autophagy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 2954-64, (2012) (PubMed).

Lu, László, Miao, Chen, Wu: "The role of nitric-oxide synthase in the regulation of UVB light-induced phosphorylation of the alpha subunit of eukaryotic initiation factor 2." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24281-8, (2009) (PubMed).

Target Details for GCN2

Target: GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))

Alternative Name: GCN2 (EIF2AK4 Products)

Background: GCN2 (EIF2AK4) belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses.

Molecular Weight: 186911

Gene ID: 440275

NCBI Accession: NP_001013725

UniProt: Q9P2K8

Pathways: ER-Nucleus Signaling, Hepatitis C