Aurora Kinase B antibody (pThr232)

Catalog No. ABIN401272

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: AA 223-234, pThr232

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. Cross reactivity with Aurora Kinase B from other sources is not known.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine, Pig, Dog and Chimpanzee (100 %).
Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000-1/50,000. Western blot: 1/250-1/2,000 (See protocol below). Expect a band approx. 39 kDa in size corresponding to Aurora Kinase B by western blotting in theappropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.21 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora Kinase B (AURKB Products)

Background: Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

Gene ID: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades