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Goat anti-Guinea Pig IgM (Fc Region) Antibody (FITC) Secondary Antibody

ELISA, ICC, IF, IHC (fro) Polyclonal
Pubmed (1)
Catalog No. ABIN457754
$479.71
Plus shipping costs $45.00
1 mL
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  • Target
    Binding Specificity
    Fc Region
    Reactivity
    Guinea Pig
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    FITC
    Application
    ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgM, Fc specific
    Cross-Reactivity (Details)
    This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.
    Characteristics
    The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against guinea pig serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
    Purification
    Purified
    Immunogen
    Purified IgM isolated from guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
    Buffer
    FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
    Preservative
    Without preservative
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
  • Lei, Tran, Wang, Steinhardt, Xiao, Chiang, Wyatt, Li: "Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs." in: Frontiers in microbiology, Vol. 10, pp. 672, 2019 (PubMed).

  • Target
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