Dinitrophenol Coupled Albumin antibody
Reactivity: Monkey
ID, IEP
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Dinitrophenol Coupled Albumin
- Reactivity
- Monkey
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- Specificity
- Inter-species cross reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however a reaction has been observed with human albumin, chicken ovalbuminDNP, but not with native chicken ovalbumin.
- Characteristics
- Precipitating polyclonal rabbit antiserum to monkey dinitrophenol coupled albumin
- Purification
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- Immunogen
- Purified albumin isolated from pooled monkey serum, coupled to dinitrophenol. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) and electroimmunodiffusion (Laurell) to identify the presence of albumin or albuminDNP in monkey serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal monkey serum in agar-block immunodiffusion titr
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Target
- Dinitrophenol Coupled Albumin
- Background
- The reactivity of the antiserum is restricted to albumin and albuminDNP as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified albumin
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