ASRGL1 antibody (Biotin)

Catalog No. ABIN459055

Product Details anti-ASRGL1 Antibody (Biotin)

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASRGL1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-asparaginase from Escherichia coli

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: L-Asparaginase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for ASRGL1

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Alternative Name: L-asparaginase (ASRGL1 Products)

Synonyms: ASRGL1 antibody, ALP antibody, ALP1 antibody, CRASH antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, Gliap antibody, Hiob2 antibody, zgc:103568 antibody, asparaginase like 1 antibody, L-asparaginase antibody, isoaspartyl peptidase/L-asparaginase antibody, ASRGL1 antibody, STY0886 antibody, ansA antibody, ECA0132 antibody, LOC5580113 antibody, EcHS_A0886 antibody, LACBIDRAFT_317700 antibody, BCAM1291 antibody, ZPR_0296 antibody, PAAG_01206 antibody, asrgl1 antibody, Asrgl1 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.