Ubiquitin antibody

Details for Product No. ABIN462018
Target
Reactivity
Cow, Human, Mouse
235
157
156
129
69
67
57
53
50
50
49
48
48
47
47
47
30
18
10
7
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
189
127
4
3
2
1
Clonality
Monoclonal
Conjugate
Un-conjugated
17
14
13
12
12
12
7
7
7
7
7
7
7
7
7
7
7
5
4
4
4
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
289
181
168
102
89
84
38
38
16
16
13
12
11
5
1
1
1
Options
Brand IHC-plus™
Immunogen Purified bovine ubiquitin conjugated to carrier protein.

Type of Immunogen: Purified protein
Isotype IgG1, kappa
Specificity Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.
Purification Protein A purified
Target
Pathways Mitotic G1-G1/S Phases
Application Notes Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl,  pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.
Comment

Target Species of Antibody: Bovine

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Images
Image no. 1 for anti-Ubiquitin antibody (ABIN462018) Human Brain, Cortex, Senile Plaque (formalin-fixed, paraffin-embedded) stained with U...
Image no. 2 for anti-Ubiquitin antibody (ABIN462018) Human Testis (formalin-fixed, paraffin-embedded) stained with Ubiquitin antibody ABIN...
Image no. 3 for anti-Ubiquitin antibody (ABIN462018) Anti-Ubiquitin antibody IHC of human brain, cortex, senile plaque. Immunohistochemist...