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Tryptase alpha/beta 1 (TPSAB1) (AA 65-275) antibody Primary Antibody

TPSAB1 Reactivity: Human ELISA, IHC (p), WB Host: Rabbit Polyclonal
Catalog No. ABIN4886754
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 65-275
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Tryptase alpha/beta-1(TPSAB1) detection. Tested with WB, IHC-P, ELISA in Human.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Tryptase alpha/beta-1(TPSAB1) detection. Tested with WB, IHC-P, ELISA in Human.
    Gene Name: tryptase alpha/beta 1
    Protein Name: Tryptase alpha/beta-1
    Purification
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.
    Isotype
    IgG
  • Target
    Alternative Name
    TPSAB1 (TPSAB1 Antibody Abstract)
    Background
    Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

    Synonyms: alpha II | Lung tryptase | MCP 7 | Pituitary tryptase | Skin tryptase | TPS 1 | TPS1 | TPS2 | TPSAB1 | TPSAB1 protein | TPSB1 | Tryptase 1 | Tryptase alpha 1 | tryptase alpha I included | Tryptase alpha II | tryptase alpha II included | tryptase alpha included | tryptase alpha/beta 1 | Tryptase beta 1 | tryptase beta I included | Tryptase I | tryptase I included | Tryptase III | Tryptase skin | Q15661
    Gene ID
    7177
    UniProt
    Q15661
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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