XIAP antibody (C-Term)

Catalog No. ABIN501135

Product Details anti-XIAP Antibody

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects HILP, API3, XIAP at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: IAP3 / BIRC4 (XIAP Products)

Background: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases (1 for review). The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases -3, -7, and -9 (2,3). Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation, conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation (4). Two negative regulators, termed XAF-1 (5) and Smac (6), can bind and inhibit XIAP activity.Synonyms: API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, Inhibitor of apoptosis protein 3, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP

Gene ID: 331

NCBI Accession: NP_001158

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis