Ubiquitin antibody
(1 reference)-
- Target See all Ubiquitin Antibodies
- Ubiquitin
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ubiquitin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.
- Purification
- Gel filtration
- Immunogen
- Ubiquitin conjugated lysozyme
- Clone
- FK1
- Isotype
- IgM
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin Reactivity: Human, Rat WB, IHC, ICC, IP Host: Mouse Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (PerCP)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal PerCP
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- Application Notes
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- WB (1:10000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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: "Rainbow Trout Red Blood Cells Exposed to Viral Hemorrhagic Septicemia Virus Up-Regulate Antigen-Processing Mechanisms and MHC I&II, CD86, and CD83 Antigen-presenting Cell Markers." in: Cells, Vol. 8, Issue 5, (2019) (PubMed).
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: "Rainbow Trout Red Blood Cells Exposed to Viral Hemorrhagic Septicemia Virus Up-Regulate Antigen-Processing Mechanisms and MHC I&II, CD86, and CD83 Antigen-presenting Cell Markers." in: Cells, Vol. 8, Issue 5, (2019) (PubMed).
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- Target
- Ubiquitin
- Alternative Name
- Ubiquitin (Ubiquitin Products)
- Background
- Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
- Gene ID
- 7314
- UniProt
- P0CG47
- Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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