Hexanoyl-Lysine Adduct (HEL) antibody (Atto 594)

Catalog No. ABIN5067239

Product Details

Target: Hexanoyl-Lysine Adduct (HEL)

Reactivity: All Species

Host: Mouse

Clonality: Monoclonal

Conjugate: Atto 594

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Purification: Protein G Purified

Immunogen: Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

Clone: 5D9

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• FACS (1:50)
• FCM (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067239 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: Hexanoyl-Lysine Adduct (HEL)

Alternative Name: Hexanoyl-Lysine adduct

Background: Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.