SPARC antibody (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin)) (AA 224-251)

Details for Product anti-SPARC Antibody No. ABIN5536420
Antigen
  • ON
  • AA517111
  • BM-40
  • hm:zeh0062
  • SPARC
  • sparc
  • secreted protein acidic and cysteine rich
  • secreted acidic cysteine rich glycoprotein
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • secreted protein, acidic, cysteine-rich (osteonectin) L homeolog
  • secreted protein, acidic, cysteine-rich (osteonectin) S homeolog
  • SPARC
  • Sparc
  • sparc
  • sparc.L
  • sparc.S
Epitope
AA 224-251, C-Term
16
16
15
11
9
6
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
181
71
62
17
7
6
5
4
4
2
1
1
1
1
Host
Rabbit
169
47
4
3
2
Clonality
Polyclonal
Conjugate
This SPARC antibody is un-conjugated
19
12
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
171
96
93
28
27
14
14
13
12
10
10
9
9
5
Options
Immunogen This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
Isotype Ig Fraction
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Plasmids, Primers & others Plasmids, Primers & others SPARC products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name SPARC (SPARC Antibody Abstract)
Background Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
Molecular Weight 35 kDa
Gene ID 6678
UniProt P09486
Pathways Autophagy
Application Notes For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Restrictions For Research Use only
Format Liquid
Buffer Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Supplier Images
Image no. 1 for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibody (ABIN5536420) Western blot analysis of SPARC Antibody in Y79 cell line lysates (35ug/lane)
Image no. 2 for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibody (ABIN5536420) Western blot analysis of SPARC using rabbit polyclonal SPARC Antibody using 293 cell ...
Image no. 3 for anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibody (ABIN5536420) Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , ...
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