DNAJC5 antibody (AA 177-191)

Catalog No. ABIN5539515

Product Details anti-DNAJC5 Antibody

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Binding Specificity: AA 177-191

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DNAJC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Dnajc5 (aa177-191)

Sequence: SATETTQLTA DSHPS

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 20 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1 kDa according to NP_058055.1). Recommended concentration: 0.5-1.5 μg/mL. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular bat

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DNAJC5

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Alternative Name: Dnajc5 (DNAJC5 Products)

Background: Dnajc5, DnaJ (Hsp40) homolog, subfamily C, member 5, 2610314I24Rik, AU018536, Csp, cysteine string protein, dnaJ homolog subfamily C member 5

Gene ID: 80331, 13002, 79130

NCBI Accession: NP_058055

Pathways: Synaptic Vesicle Exocytosis