DLD antibody (HRP)

Catalog No. ABIN5596706

Supplier Product No.: 200-4360-0100

Product Details anti-DLD Antibody (HRP)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-4360-0100

Supplier: Rockland

Purpose: Lipoamide Dehydrogenase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Characteristics: Synonyms: rabbit anti-Lipoamide Dehydrogenase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase

Purification: Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Lipoamide Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: DLD LAD (DLD Products)

Background: Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Gene ID: 397129, 118675

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis