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HMW HCP antibody

HMW HCP Reactivity: E. coli ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5596741
  • Target
    HMW HCP (HMW Host Cell Proteins (HMW HCP))
    Reactivity
    E. coli
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This HMW HCP antibody is un-conjugated
    Application
    ELISA, Western Blotting (WB)
    Purpose
    Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.
    Cross-Reactivity (Details)
    Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
    Characteristics

    Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

    Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

    Purification
    This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).

    Immunogen Type: Native Protein

  • Application Notes

    Western Blot Dilution: 1:500 - 1:1000

    ELISA Dilution: 1:1000-1:2000

    Comment

    Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    77.5 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store HMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    HMW HCP (HMW Host Cell Proteins (HMW HCP))
    Alternative Name
    HMW E.Coli Host Cell Proteins
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