HMW Host Cell Proteins (HMW HCP) antibody

Details for Product No. ABIN5596741
Antigen
Reactivity
Escherichia coli (E. coli)
1
Host
Rabbit
1
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
1
1
Options
Purpose Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.
Immunogen

Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Cross-Reactivity (Details) Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Characteristics

Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Purification This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
Sterility Sterile filtered
Antigen
Alternative Name HMW E.Coli Host Cell Proteins
Application Notes

Western Blot Dilution: 1:500 - 1:1000

ELISA Dilution: 1:1000-1:2000

Comment

Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

Restrictions For Research Use only
Format Liquid
Concentration 77.5 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store HMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-HMW Host Cell Proteins (HMW HCP) antibody (ABIN5596741) 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load...
ELISA image for anti-HMW Host Cell Proteins (HMW HCP) antibody (ABIN5596741) ELISA of Rabbit anti-High Molecular Weight Host Cell Protein antibody. Antigen: BSA c...
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