Collagen, Type I (COL1) antibody

Details for Product No. ABIN5596819
Cow, Human, Mammalian, Mouse, Rat
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Immunogen: Collagen Type I from human and bovine placenta

Immunogen Type: Native Protein

Isotype IgG
Cross-Reactivity (Details) Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Purification COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Alternative Name COLLAGEN Type I

Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL1A1

Gene ID 1277
UniProt P02452
Application Notes

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months
Image no. 1 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Age-related alterations in collagen ...
Image no. 2 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) CCR2 mediates the specific accumulation ...
Image no. 3 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Oridonin suppresses α-SMA and ECM prote...
Image no. 4 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) CYD0692 suppresses endogenous and TGF-β...
Image no. 5 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) The testing of multi-differentiation of r...
Image no. 6 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) CYD0682 suppresses endogenous ECM prote...
Image no. 7 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Collagen type I expression in the IVD tissue. A...
Image no. 8 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) At least two grid per tissue biopsies were pr...
Image no. 9 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Effect of ex vivo treatment with Pirfen...
Image no. 10 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Rabbit Anti-collagen type I antibody. Tissue: right lobe of t...
Image no. 11 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunofluorescence and SHG detection...
Image no. 12 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Inhibition of mTORC1 reduces secretion of type ...
Image no. 13 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) p21 knockout alleviates liver fibrosis. A...
Image no. 14 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Decreased gene and protein expression ...
Image no. 15 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Representative histological analysis for...
Image no. 16 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) ASH1 positively influences the expression of TI...
Image no. 17 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) AICAR treatment reduces post-MI adver...
Image no. 18 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) SMT022357 improves the pathology in the diaphr...
Image no. 19 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) The augmented participation from infilt...
Image no. 20 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to d...
Image no. 21 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Reduced secretion of type I collagen by Akt inh...
Image no. 22 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Anti-fibrotic effect of apigenin in human HSC l...
Image no. 23 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) LPA increases α-SMA and ECM production in H...
Image no. 24 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Histological analysis of muscle and tend...
Image no. 25 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Both serum-free and serum-containing...
Image no. 26 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunoperoxidase stain for collagen type I (...
Image no. 27 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Collagen Far Western blot of B. frag...
Image no. 28 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) SIM mutant as dominant negative prot...
Image no. 29 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Transgenic VEGFA overexpression reduced...
Image no. 30 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formali...
Image no. 31 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fi...
Image no. 32 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Zoledronic acid (ZA) at the maximum concen...
Image no. 33 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) HJC0123 inhibits hepatic stellate cells activa...
Image no. 34 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Recombinant ACLP inhibits adipogenes...
Image no. 35 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. L...
Image no. 36 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Expression of collagen by 5 day culture...
Image no. 37 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Pulmonary fibroblasts deposit elastin ...
Image no. 38 for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Inhibition of collagen prolyl hydroxylase by PD...
Product cited in: Ho, Skiba, Ullmer, Rao: "Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells." in: Investigative ophthalmology & visual science, Vol. 59, Issue 5, pp. 1969-1984, 2019 (PubMed).

Surolia, Li, Wang, Li, Liu, Zhou, Luckhardt, Bae, Liu, Rangarajan, de Andrade, Thannickal, Antony: "3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs." in: JCI insight, Vol. 2, Issue 2, pp. e91377, 2019 (PubMed).

Bürgi, Kunz, Abrami, Deuquet, Piersigilli, Scholl-Bürgi, Lausch, Unger, Superti-Furga, Bonaldo, van der Goot: "CMG2/ANTXR2 regulates extracellular collagen VI which accumulates in hyaline fibromatosis syndrome." in: Nature communications, Vol. 8, pp. 15861, 2018 (PubMed).

Zhang, Stefanovic: "mTORC1 phosphorylates LARP6 to stimulate type I collagen expression." in: Scientific reports, Vol. 7, pp. 41173, 2018 (PubMed).

Hicks, Goossens, Blas-García, Tsuchida, Wooden, Wallace, Nieto, Lade, Redhead, Cederbaum, Dudley, Fuchs, Lee, Hoshida, Friedman: "Transcriptome-based repurposing of apigenin as a potential anti-fibrotic agent targeting hepatic stellate cells." in: Scientific reports, Vol. 7, pp. 42563, 2018 (PubMed).

Trial, Heredia, Taffet, Entman, Cieslik: "Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis." in: Basic research in cardiology, Vol. 112, Issue 4, pp. 34, 2018 (PubMed).

Cummins, Wang, Nunez Lopez, Graham, Tie, Zhou, Radhakrishnan: "Luteolin-Mediated Inhibition of Hepatic Stellate Cell Activation via Suppression of the STAT3 Pathway." in: International journal of molecular sciences, Vol. 19, Issue 6, 2018 (PubMed).

Lehmann, Buhl, Alsafadi, Klee, Hermann, Mutze, Ota, Lindner, Behr, Hilgendorff, Wagner, Königshoff: "Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 175, 2018 (PubMed).

Jager, Lee, Li, Farmer, Fried, Layne: "Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue." in: PLoS ONE, Vol. 13, Issue 5, pp. e0197777, 2018 (PubMed).

Laternser, Keller, Leupin, Rausch, Graf-Hausner, Rimann: "A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues." in: SLAS technology, Vol. 23, Issue 6, pp. 599-613, 2018 (PubMed).

Shukunami, Takimoto, Nishizaki, Yoshimoto, Tanaka, Miura, Watanabe, Sakuma, Yamamoto, Kondoh, Hiraki: "Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 3155, 2018 (PubMed).

Roselló-Díez, Stephen, Joyner: "Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth." in: eLife, Vol. 6, 2018 (PubMed).

He, Bougioukli, Ortega, Arevalo, Lieberman, McMahon: "Sox9 positive periosteal cells in fracture repair of the adult mammalian long bone." in: Bone, Vol. 103, pp. 12-19, 2018 (PubMed).

Zhang, Stefanovic: "Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen." in: Scientific reports, Vol. 6, pp. 22597, 2017 (PubMed).

Komatsu, Ibi, Chosa, Kyakumoto, Kamo, Shibata, Sugiyama, Ishisaki: "Zoledronic acid suppresses transforming growth factor-β-induced fibrogenesis by human gingival fibroblasts." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 139-47, 2017 (PubMed).

Murray, Habiel, Hohmann, Camelo, Shang, Zhou, Coelho, Peng, Gulati, Crestani, Sleeman, Mustelin, Moore, Ryu, Osafo-Addo, Elias, Lee, Hu, Herazo-Maya, Knight, Hogaboam, Herzog: "Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis." in: JCI insight, Vol. 2, Issue 16, 2017 (PubMed).

Yang, Peng, Gopalan, Gao, Chen, Joyner: "Stromal hedgehog signaling maintains smooth muscle and hampers micro-invasive prostate cancer." in: Disease models & mechanisms, Vol. 10, Issue 1, pp. 39-52, 2017 (PubMed).

Yosef, Pilpel, Papismadov, Gal, Ovadya, Vadai, Miller, Porat, Ben-Dor, Krizhanovsky: "p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling." in: The EMBO journal, Vol. 36, Issue 15, pp. 2280-2295, 2017 (PubMed).

Clevenger, Hinman, Ashley Rubin, Smither, Burke, Hawker, Messina, Van Epps, Clegg: "Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells." in: Tissue engineering. Part A, Vol. 22, Issue 7-8, pp. 597-609, 2017 (PubMed).

Nunez Lopez, Bohanon, Wang, Ye, Corsello, Rojas-Khalil, Chen, Chen, Zhou, Radhakrishnan: "STAT3 Inhibition Suppresses Hepatic Stellate Cell Fibrogenesis: HJC0123, a Potential Therapeutic Agent for Liver Fibrosis." in: RSC advances, Vol. 6, Issue 102, pp. 100652-100663, 2016 (PubMed).

'Independent Validation' Badge
Antigen COL1
Lot Number 41897
Method validated Unfolding Profile
Positive Control ABIN5596819

Passed. ABIN5596819 showed Ti at 79.6°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

  • Dilute ABIN5596819 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validation Images
Unfolding Profile validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819) Unfolding profile of ABIN5596819. The fluorescence signal is plotted against temperat...