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Collagen, Type I (COL1) antibody Primary Antibody

COL1 Reactivity: Cow, Human, Mammalian, Mouse, Rat ELISA, IHC, IP, WB Host: Rabbit Polyclonal
Pubmed (62) Independent Validation (1)
Catalog No. ABIN5596819
$625.50
Plus shipping costs $45.00
100 μg ABIN5596819
100 μg ABIN5596819
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  • Target
    Reactivity
    Cow, Human, Mammalian, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Cross-Reactivity (Details)
    Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Purification
    COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: Collagen Type I from human and bovine placenta

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:50,000

    Restrictions
    For Research Use only
  • Validation #103813 (Unfolding Profile)
    'Independent Validation' Badge
    by
    NanoTemper Technologies
    No.
    #103813
    Date
    07/23/2019
    Antigen
    COL1
    Lot Number
    41897
    Method validated
    Unfolding Profile
    Positive Control
    ABIN5596819
    Negative Control
    Notes

    Passed. ABIN5596819 showed Ti at 79.6°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    Secondary Antibody
    Full Protocol
    • Dilute ABIN5596819 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Experimental Notes

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Keller, Bruch, Schneider, Meier-Hubberten, Hafner, Rudolf: "A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro." in: Cells, Vol. 9, Issue 8, 2020 (PubMed).

    Thomas, Ahangar, Hofma, Strudwick, Fitridge, Mills, Cowin: "Attenuation of Flightless I Increases Human Pericyte Proliferation, Migration and Angiogenic Functions and Improves Healing in Murine Diabetic Wounds." in: International journal of molecular sciences, Vol. 21, Issue 16, 2020 (PubMed).

    Roth, Enström, Aghabeick, Carlsson, Genové, Paul: "Parenchymal pericytes are not the major contributor of extracellular matrix in the fibrotic scar after stroke in male mice." in: Journal of neuroscience research, Vol. 98, Issue 5, pp. 826-842, 2020 (PubMed).

    Rigon, Hörner, Straka, Bieback, Gretz, Hafner, Rudolf: "Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage." in: Frontiers in molecular neuroscience, Vol. 13, pp. 107, 2020 (PubMed).

    Fujiwara, Funaki, Fukui, Kimura, Kanou, Ose, Minami, Shintani: "Effects of pirfenidone targeting the tumor microenvironment and tumor-stroma interaction as a novel treatment for non-small cell lung cancer." in: Scientific reports, Vol. 10, Issue 1, pp. 10900, 2020 (PubMed).

    Kramer, Aigner, Petzold, Roshanbinfar, Scheibel, Engel: "Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering." in: Scientific reports, Vol. 10, Issue 1, pp. 8789, 2020 (PubMed).

    Kumar Gupta, Sarkar, Wertheim, Pan, Carroll, Oxburgh: "Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids." in: Communications biology, Vol. 3, Issue 1, pp. 231, 2020 (PubMed).

    Khan, Sankaran, Llontop, Del Campo: "Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro." in: BMC molecular and cell biology, Vol. 21, Issue 1, pp. 22, 2020 (PubMed).

    Ho, Skiba, Ullmer, Rao: "Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells." in: Investigative ophthalmology & visual science, Vol. 59, Issue 5, pp. 1969-1984, 2019 (PubMed).

    Surolia, Li, Wang, Li, Liu, Zhou, Luckhardt, Bae, Liu, Rangarajan, de Andrade, Thannickal, Antony: "3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs." in: JCI insight, Vol. 2, Issue 2, pp. e91377, 2019 (PubMed).

    Fujii, Taga, Sakai, Ito, Hattori, Nagata, Koide: "Lowering the culture temperature corrects collagen abnormalities caused by HSP47 gene knockout." in: Scientific reports, Vol. 9, Issue 1, pp. 17433, 2019 (PubMed).

    Nettesheim, Shim, Hirt, Liton: "Transcriptome analysis reveals autophagy as regulator of TGFβ/Smad-induced fibrogenesis in trabecular meshwork cells." in: Scientific reports, Vol. 9, Issue 1, pp. 16092, 2019 (PubMed).

    Ashok, Kang, Wise, Pattabiraman, Johnson, Lonigro, Ravikumar, Rhee, Singh: "Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma." in: Scientific reports, Vol. 9, Issue 1, pp. 13090, 2019 (PubMed).

    Cordes, Ney, Beleites, Aust, Baretton, Thames, Baumann, Krause, Löck, Appold: "Retrospective investigation of the prognostic value of the β1 integrin expression in patients with head and neck squamous cell carcinoma receiving primary radio(chemo)therapy." in: PLoS ONE, Vol. 13, Issue 12, pp. e0209479, 2019 (PubMed).

    Wu, Dao Thi, Huang, Billerbeck, Saha, Hoffmann, Wang, Silva, Sarbanes, Sun, Andrus, Yu, Quirk, Li, MacDonald, Schneider, An, Rosenberg, Rice: "Intrinsic Immunity Shapes Viral Resistance of Stem Cells." in: Cell, Vol. 172, Issue 3, pp. 423-438.e25, 2019 (PubMed).

    Bürgi, Kunz, Abrami, Deuquet, Piersigilli, Scholl-Bürgi, Lausch, Unger, Superti-Furga, Bonaldo, van der Goot: "CMG2/ANTXR2 regulates extracellular collagen VI which accumulates in hyaline fibromatosis syndrome." in: Nature communications, Vol. 8, pp. 15861, 2018 (PubMed).

    Zhang, Stefanovic: "mTORC1 phosphorylates LARP6 to stimulate type I collagen expression." in: Scientific reports, Vol. 7, pp. 41173, 2018 (PubMed).

    Hicks, Goossens, Blas-García, Tsuchida, Wooden, Wallace, Nieto, Lade, Redhead, Cederbaum, Dudley, Fuchs, Lee, Hoshida, Friedman: "Transcriptome-based repurposing of apigenin as a potential anti-fibrotic agent targeting hepatic stellate cells." in: Scientific reports, Vol. 7, pp. 42563, 2018 (PubMed).

    Trial, Heredia, Taffet, Entman, Cieslik: "Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis." in: Basic research in cardiology, Vol. 112, Issue 4, pp. 34, 2018 (PubMed).

    Cummins, Wang, Nunez Lopez, Graham, Tie, Zhou, Radhakrishnan: "Luteolin-Mediated Inhibition of Hepatic Stellate Cell Activation via Suppression of the STAT3 Pathway." in: International journal of molecular sciences, Vol. 19, Issue 6, 2018 (PubMed).

  • Target
    Alternative Name
    COLLAGEN Type I
    Background

    Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

    Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

    Gene Name: COL1A1

    Gene ID
    1277
    UniProt
    P02452
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