COL2 antibody (Collagen, Type II)

Details for Product anti-COL2 Antibody No. ABIN5596825
Antigen
  • PT
  • RPRGL2
  • THPH1
  • Cf-2
  • Cf2
  • FII
  • thrombin
  • prothrombin
  • coagulation factor II, thrombin
  • coagulation factor II
  • F2
Reactivity
Cow (Bovine), Human, Mammalian, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
115
83
71
36
33
22
7
4
3
2
1
1
Host
Rabbit
119
50
22
7
Clonality
Polyclonal
Conjugate
This COL2 antibody is un-conjugated
28
8
4
4
4
4
4
4
3
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
112
104
85
49
46
37
24
15
13
12
11
10
7
6
6
2
2
2
2
1
1
Options
Immunogen

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein
Isotype IgG
Cross-Reactivity (Details) Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Purification Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Antigen
Alternative Name Collagen Type II (COL2 Antibody Abstract)
Background

Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody

Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL2A1
Gene ID 1280
UniProt P02458
Application Notes

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Collagen, Type II (COL2) antibody (ABIN5596825) anti collagen II antibody (600-401-104 Lot 26014, 1:400, 45 min RT) showed moderate s...
Product cited in: Kondo, Shukunami, Morioka, Matsumoto, Takahashi, Oh, Atsumi, Umezawa, Kudo, Kitayama, Hiraki, Noda: "Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells." in: Journal of cell science, Vol. 120, Issue Pt 5, pp. 849-57, 2007 (PubMed).

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