COL2A1 antibody

Catalog No. ABIN5596825

Supplier Product No.: 600-401-104-0.5

Product Details anti-COL2A1 Antibody

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 600-401-104-0.5

Supplier: Rockland

Purpose: Collagen Type II Antibody

Cross-Reactivity (Details): Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Purification: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Sterility: Sterile filtered

Immunogen:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References for anti-COL2A1 antibody (ABIN5596825)

Kondo, Shukunami, Morioka, Matsumoto, Takahashi, Oh, Atsumi, Umezawa, Kudo, Kitayama, Hiraki, Noda: "Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells." in: Journal of cell science, Vol. 120, Issue Pt 5, pp. 849-57, (2007) (PubMed).

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2A1 (COL2A1 Products)

Background: Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene ID: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding