COL3A1 antibody

Catalog No. ABIN5596829

Supplier Product No.: 600-401-105-0.5

Product Details anti-COL3A1 Antibody

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivity: Human, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-105-0.5

Supplier: Rockland

Purpose: Collagen Type III Antibody

Cross-Reactivity (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

Purification: Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.

Sterility: Sterile filtered

Immunogen:

Immunogen: Collagen Type III from human and bovine placenta

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Collagen Type III has been tested by dot Blot, western blot, and IHC and is useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-COL3A1 antibody (ABIN5596829)

Chakraborty, Wirrig, Hinton, Merrill, Spicer, Yutzey: "Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves." in: Developmental biology, Vol. 347, Issue 1, pp. 167-79, (2010) (PubMed).

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Alternative Name: COL3A1 (COL3A1 Products)

Background: Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Gene ID: 1281

NCBI Accession: NP_000081

UniProt: P02461

Pathways: Autophagy, Growth Factor Binding