Collagen, Type III (COL3) antibody

Details for Product No. ABIN5596829
Target
Reactivity
Cow, Human, Mammalian
94
48
44
11
5
2
1
Host
Rabbit
72
19
16
Clonality
Polyclonal
Conjugate
Un-conjugated
9
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
62
49
46
26
24
22
22
16
15
8
6
6
3
3
2
2
1
1
1
1
Options
Immunogen

Immunogen: Collagen Type III from human and bovine placenta

Immunogen Type: Native Protein

Isotype IgG
Cross-Reactivity (Details) Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Purification Anti-Collagen Type III has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Target
Alternative Name COLLAGEN Type III (COL3 Antibody Abstract)
Background

Synonyms: Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody

Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Gene Name: COL3A1

Gene ID 1281
UniProt P02461
Application Notes

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

Restrictions For Research Use only
Format Liquid
Reconstitution

Reconstitution Volume: 500 μL

Concentration 1.0 mg/mL
Buffer

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months
Images
Image no. 1 for anti-Collagen, Type III (COL3) antibody (ABIN5596829) anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong st...
Image no. 2 for anti-Collagen, Type III (COL3) antibody (ABIN5596829) Immunohistochemistry of Rabbit Anti-collagen type III antibody. Tissue: right lobe of...
Image no. 3 for anti-Collagen, Type III (COL3) antibody (ABIN5596829) Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 10...
Product cited in: Chakraborty, Wirrig, Hinton, Merrill, Spicer, Yutzey: "Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves." in: Developmental biology, Vol. 347, Issue 1, pp. 167-79, 2010 (PubMed).