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Urate Oxidase (UOX) antibody (HRP) Primary Antibody

UOX Reactivity: Bacillus ELISA, IP, WB Host: Goat Polyclonal
Catalog No. ABIN5596984
$625.50
Plus shipping costs $45.00
100 μg
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  • Target
    Reactivity
    Bacillus
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Application
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    Cross-Reactivity (Details)
    Cross reactivity against Uricase from other sources is unknown.
    Purification
    Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].
    Immunogen

    Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Uricase antibody is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1,000 - 1:2,500

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Alternative Name
    Uricase (UOX Antibody Abstract)
    Background

    Synonyms: Urate oxidase antibody

    Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

    Gene Name: ABC3735

    Gene ID
    3202700
    UniProt
    Q5WBJ3
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