This Glucose-6-Phosphate Dehydrogenase antibody is conjugated to Biotin
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
Characteristics
Molar Ratio: Biotin/ IgG ~4.7
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Glucose-6-Phosphate Dehydrogenase isolated and purified from Leuconostoc mesenteroides. Freund’s complete adjuvant is used in the first step of the immunization procedure.
G6PD
Reactivity: Human, Mouse, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase