Topoisomerase I antibody (AA 526-765)
-
- Target See all Topoisomerase I (TOP1) Antibodies
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
-
Binding Specificity
- AA 526-765
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Topoisomerase I antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for Topoisomerase I detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.
- Immunogen
- E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765).
- Top Product
- Discover our top product TOP1 Primary Antibody
-
-
- Application Notes
-
Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Alternative Name
- TOP1 (TOP1 Products)
- Target Type
- Viral Protein
- Background
-
Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1,
Tissue Specificity: Endothelial cells.
Background: DNA topoisomerase 1 is an enzyme that in humans is encoded by the TOP1 gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
- UniProt
- P11387
- Pathways
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
-