PI4K alpha Type I (Internal Region) antibody
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- Target
- PI4K alpha Type I
- Binding Specificity
- Internal Region
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 600-401-j91
- Supplier
- Rockland
- Purpose
- PI4K alpha type I Antibody
- Cross-Reactivity (Details)
- A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.
- Characteristics
- Synonyms: rabbit anti-PI4K alpha antibody, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, PIP5KIalpha, PIP5K1A
- Purification
- Anti-PI4K, type I alpha was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Anti-PI4K, type I alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-PI4K alpha antibody is tested by IHC and ELISA useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.6 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: None - Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PI4K alpha Type I
- Background
- Background: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha is a member of PI3/PI4-kinase family, PI4K alpha is expressed with the highest amount of expression in the heart, placenta, skeletal muscle, kidney and pancreas. This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Anti-PI4K alpha antibody is ideal for investigators interested in Kinase and Phosphatase Antibodies.
- UniProt
- Q99755
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