L-Cysteine antibody
IA, IP, IHC, WB
Host: Mouse
Monoclonal
F2D
unconjugated
1 image
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- Target
- L-Cysteine
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunoassay (IA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
- Brand
- AbX™
- Characteristics
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Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein
For use under Non-Reducing conditions - Purification
- Protein A Purified
- Immunogen
- Cysteine conjugated to Keyhole Limpet Hemocyanin
- Clone
- F2D
- Isotype
- IgG2a
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- Application Notes
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Western blotting, Immunoassay, Immunohistochemistry and Immunoprecipitation
Western blotting, 1:1,000 AbX™ Anti-Cysteine Mouse Monoclonal ImmuNOhIstOChemIstry Porcine peripheral red blood cells were paraffin embedded and sectioned. 4 μm sections were de-paraffinized and rehydrated. Sections were incubated with or without additional thiol compounds and anti-cysteine anti- body for 2 hours at room temperature. Antibody concentration was 3 μg/mL. Sections were blocked pre- and post antibody treatements with 1 % BSA in PBS. Development was with a goat anti-mouse IgG antibody labeled with Alexa™ 488. RBC + Anti-Cysteine RBC + Anti-Cysteine + 20 mM Cysteine Cysteine addition was carried out at 20 mM in 1 % BSA in PBS and added with the monoclonal anti-cysteine antibody at 3 μg/mL for 1 hour at room temperature. With polymorphonuclear neutrophils addition of 20 mM cytseine reduced or eliminated the goat anti-mouse IgG-Alexa 488 signal PMN + Anti-Cysteine PMN + Anti-Cysteine + 20 mM Cysteine Nuclear staining was carried out with TOPRO at 1:500 dilution in PBS. - Comment
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Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein For use under Non-Reducing conditions
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C
- Storage Comment
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- Target
- L-Cysteine
- Target Type
- Amino Acid
- Background
- Among several postranslational modifications known to date, cysteinylation has received relatively very little interest. Cysteinylation has been implicated in the regulation of immunity, protein kinase C activity and as a marker for utero-placental insufficiency as monitored by serum albumin cysteinylation. Protein S-thiolation by low molecular weight (LMW) thiols prevents the irreversible oxidation of cysteine residues during oxidative stress and plays a role in the redox regulation of thiol-containing proteins. Many Gram-positive bacteria lack glutathione and so the nature of S-thiolation in these organisms remains elusive. In the Gram-positive model organism Bacil- lus subtilis, cysteine represents the most abundant LMW thiol. One of the most obvious responses of B. subtilis to oxidative stress is the strong induction of cysteine biosynthesis genes. Although the origins of this effect are unclear, it may be a reflection of consumption of free cysteine by oxidation to cystine and the formation of mixed disulfides with proteins. The Arbor Assays AbX™ Cysteine Mouse Monoclonal is produced as a Protein A purified antibody. It will measure cysteine-protein complexes under non-reducing conditions.
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