TIMP2 antibody (N-Term)

Catalog No. ABIN6165498

Product Details anti-TIMP2 Antibody

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Binding Specificity: AA 56-66, N-Term

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-TIMP2 (3A4), Purified, BSA-free

Characteristics: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Clone: 3A4

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2 (TIMP2 Products)

Background: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Molecular Weight: 21 kDa

Gene ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process