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Keratin 10 antibody (KRT10) (CF®568) Primary Antibody

KRT10 Reactivity: Human FACS, IF, IHC Host: Mouse Monoclonal KRT10-844 KRT10-1275 CF®568
Catalog No. ABIN6172336
$270.77
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  • Target See all Keratin 10 (KRT10) Antibodies
    Keratin 10 (KRT10)
    Reactivity
    • 153
    • 72
    • 54
    • 41
    • 31
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 82
    • 72
    • 3
    Mouse
    Clonality
    • 88
    • 69
    Monoclonal
    Conjugate
    • 86
    • 11
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 10 antibody is conjugated to CF®568
    Application
    • 80
    • 79
    • 74
    • 57
    • 39
    • 33
    • 17
    • 17
    • 16
    • 14
    • 14
    • 13
    • 3
    • 2
    • 2
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Purpose
    Mouse Monoclonal anti-Cytokeratin 10 (KRT10/844 + KRT10/1275), CF568 Conjugate
    Characteristics
    This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human KRT10 protein
    Clone
    KRT10-844 KRT10-1275
    Isotype
    IgG1 kappa
  • Application Notes
    Flow cytometry 0.5-1 μg/million cells in 0.1 mL
    • Immunohistology (formalin) 0.1-0.2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    A431, HeLa, MCF7 cells. Esophagus or tonsil.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Keratin 10 (KRT10)
    Alternative Name
    Cytokeratin 10 (KRT10 Products)
    Synonyms
    BCIE, BIE, CK10, EHK, K10, KPP, D130054E02Rik, K1C1, Krt-1.10, Krt1-10, Ka10, Ker10, KRT10, KRT25, KRT27, KRT28, keratin 10, keratin 10, type I S homeolog, keratin, type I cytoskeletal 25, KRT10, Krt10, krt10.S, LOC101085587
    Background
    BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
    Molecular Weight
    56.5 kDa
    Gene ID
    3858, 99936
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