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MLANA antibody (Melan A) (CF®405S) Primary Antibody

MLANA Reactivity: Human, Mouse, Rat FACS, IF, IHC, ISt, WB Host: Rabbit Monoclonal MLANA-1409R CF®405S
Catalog No. ABIN6173462
$270.77
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  • Target See all MLANA Antibodies
    MLANA
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 250
    • 161
    • 139
    • 58
    • 24
    Human, Mouse, Rat
    Host
    • 136
    • 114
    • 1
    Rabbit
    Clonality
    • 169
    • 82
    Monoclonal
    Conjugate
    • 86
    • 15
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This MLANA antibody is conjugated to CF®405S
    Application
    • 191
    • 164
    • 158
    • 139
    • 94
    • 76
    • 34
    • 26
    • 26
    • 25
    • 17
    • 16
    • 8
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Western Blotting (WB)
    Purpose
    Recombinant Rabbit Monoclonal anti-MART-1 / Melan-A / MLANA (MLANA/1409R), CF405S Conjugate
    Characteristics
    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human full-length MLANA protein
    Clone
    MLANA-1409R
    Isotype
    IgG kappa
  • Application Notes
    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0 for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    MLANA
    Alternative Name
    MART-1 / Melan-A / MLANA (MLANA Products)
    Synonyms
    A930034P04Rik, Mart1, MART-1, MART1, melan-A, Mlana, MLANA
    Background
    Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
    Molecular Weight
    20-22 kDa (doublet)
    Gene ID
    2315, 154069
    UniProt
    Q16655
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