Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD16 antibody (CD16) (CF®647) Primary Antibody

CD16 Reactivity: Human FACS, IF Host: Mouse Monoclonal C16-1045 CF®647
Catalog No. ABIN6173546
$270.77
Plus shipping costs $45.00
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target See all CD16 Antibodies
    CD16
    Reactivity
    • 153
    • 37
    • 11
    • 10
    • 10
    • 9
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 141
    • 29
    • 23
    • 1
    Mouse
    Clonality
    • 173
    • 20
    Monoclonal
    Conjugate
    • 70
    • 23
    • 17
    • 15
    • 10
    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD16 antibody is conjugated to CF®647
    Application
    • 170
    • 47
    • 42
    • 42
    • 30
    • 29
    • 20
    • 16
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    Mouse Monoclonal anti-CD16 (C16/1045), CF647 Conjugate
    Characteristics
    This antibody recognizes CD16 (FcγRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcγRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human CD16 protein
    Clone
    C16-1045
    Isotype
    IgG1 kappa
  • Application Notes
    Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    K-562 or U-937 cells. Lymph nodes and tonsils.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    CD16
    Alternative Name
    CD16 (CD16 Products)
    Synonyms
    Fcgr3, Fcgr3a, CD16, FCGR3, FCGR3@, CD16b, FCG3, FCR-10, FCRIII, FCRIIIb, FcRIII, Fc fragment of IgG, low affinity IIa, receptor, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG receptor IIIb, Fc fragment of IgG receptor IIIa, Fc receptor, IgG, low affinity III, Fc-gamma RIII, Fcgr2a, FCGR3A, FCGR3B, Fcgr3, FCGR3
    Molecular Weight
    50-80 kDa
    Gene ID
    2214, 372679
    UniProt
    P08637
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
You are here:
help Support