YWHAZ antibody (Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (pSer58)

Details for Product anti-YWHAZ Antibody No. ABIN6256377
Target
Binding Specificity
pSer58
23
11
9
9
9
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
136
98
78
19
18
15
15
10
6
6
6
5
4
3
2
2
1
1
Host
Rabbit
128
11
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
9
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
114
69
59
30
30
13
13
13
4
4
3
3
3
Options
Immunogen A synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Serine 58
Isotype IgG
Specificity Phospho-14-3-3 zeta (Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Serine 58
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Alternative Name 14-3-3 zeta (YWHAZ Antibody Abstract)
Background

Description: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Gene: YWHAZ

Molecular Weight 28kDa
Gene ID 7534
UniProt P63104
Pathways Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Images
Image no. 1 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN6256377) Western blot analysis of 14-3-3 zeta phosphorylation expression in UV treated NIH-3T3...
Image no. 2 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN6256377) Western blot analysis of Phospho-14-3-3 zeta (Ser58) expression in various lysates
Image no. 3 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN6256377) ABIN6267565 staining HeLa cells treated with PMA 125ng/ml 30' by ICC/IF. Cells were f...
Image no. 4 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN6256377) ABIN6267565 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabili...
Image no. 5 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (pSer58) antibody (ABIN6256377) ABIN6267565 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. The ti...