POLD4 antibody (Internal Region)

Catalog No. ABIN6264334

Product Details anti-POLD4 Antibody

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: POLD4 Antibody detects endogenous levels of total POLD4.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLD4, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLD4

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Alternative Name: POLD4 (POLD4 Products)

Synonyms: 2410012M21Rik antibody, AI463381 antibody, AW060307 antibody, Polds antibody, p12 antibody, POLDS antibody, DNA polymerase delta 4, accessory subunit antibody, polymerase (DNA-directed), delta 4 antibody, POLD4 antibody, Pold4 antibody

Background:

Description: As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).

Gene: POLD4

Molecular Weight: 12 kDa

Gene ID: 57804

UniProt: Q9HCU8

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA