CD53 antibody (CD53)

Details for Product anti-CD53 Antibody No. ABIN6297690
Antigen
  • AI323659
  • Ox-44
  • Tspan25
  • MOX44
  • TSPAN25
  • OX44
  • CD53 antigen
  • CD53 molecule
  • Cd53 molecule
  • Cd53
  • CD53
Reactivity
Human
187
16
9
6
2
Host
Mouse
159
36
1
Clonality
Monoclonal
Conjugate
This CD53 antibody is un-conjugated
25
21
11
7
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunofluorescence (IF)
158
58
55
45
29
20
18
13
12
9
4
3
2
Options
Purpose Mouse anti-Human CD53 Antibody [Sodium Azide Free]
Immunogen Stimulated human leukocytes were used as the immunogen for the CD53 antibody.
Specificity Cell surface
Purification Stimulated human leukocytes were used as the immunogen for the CD53 antibody.
Antigen
Alternative Name Leukocyte surface antigen CD53 (CD53 Antibody Abstract)
Background

Target Description: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This mAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Gene Symbol: CD53///MOX44///TSPAN25

Gene ID 963
UniProt P19397
Application Notes Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.