CD44 antibody (CD44)

Details for Product anti-CD44 Antibody No. ABIN6297699
Target
Reactivity
Baboon, Green Monkey, Human
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Host
Mouse
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Clonality
Monoclonal
Conjugate
This CD44 antibody is un-conjugated
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Application
Coating (Coat), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Purpose Mouse anti-Human/Baboon/Green Monkey HCAM Antibody CD44 [Sodium Azide Free]
Immunogen Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Specificity Cell surface
Purification Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Target
Alternative Name CD44 antigen (CD44 Antibody Abstract)
Background

Target Description: This antibody recognizes a cell surface glycoprotein of 80-95 kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Gene Symbol: CD44///LHR///MDU2///MDU3///MIC4

Gene ID 960
UniProt P16070
Pathways Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
Application Notes ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.