Nucleolin antibody
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- Target See all Nucleolin (NCL) Antibodies
- Nucleolin (NCL)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Nucleolin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse anti-Human NCL Antibody [Sodium Azide Free]
- Specificity
- Nucleoli
- Purification
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Immunogen
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Top Product
- Discover our top product NCL Primary Antibody
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human WB, IHC, IF, FACS, ISt Host: Mouse Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human WB, IF, FACS, IHC (p) Host: Mouse Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil WB, IF, FACS, IHC (p) Host: Mouse Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil WB, IF, FACS, IHC (p) Host: Mouse Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 428-455) antibodyNCL Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17464 unconjugated
anti-Nucleolin (NCL) (C-Term) antibodyNCL Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse WB, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Buffer
- In 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Target
- Nucleolin (NCL)
- Alternative Name
- Nucleolin (NCL Products)
- Background
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Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Gene Symbol: NCL
- Gene ID
- 4691
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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