Nucleolin antibody (NCL)

Details for Product anti-NCL Antibody No. ABIN6297721
Antigen
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
239
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Mouse
130
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
219
140
132
97
72
60
43
25
21
17
13
9
9
2
2
1
1
Options
Purpose Mouse anti-Human NCL Antibody [Sodium Azide Free]
Immunogen SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
Specificity Nucleoli
Purification SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
Plasmids, Primers & others Plasmids, Primers & others Nucleolin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background

Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Gene Symbol: NCL
Gene ID 4691
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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