Nucleolin antibody (NCL)

Details for Product anti-NCL Antibody No. ABIN3025707
Antigen
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Alternatives
anti-Human Nucleolin antibody for Immunocytochemistry
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
237
61
37
11
11
10
9
9
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3
1
1
1
1
1
1
1
Host
Mouse
130
114
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
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1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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138
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97
72
58
38
24
21
17
13
9
9
2
1
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1
Options
Immunogen Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clone NCL-902
Isotype IgG1
Purification Protein G affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others Nucleolin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes Optimal dilution of the Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Supplier Images
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry of human Jurkat cells. Grey: isotype control; Green: FITC-labeled; Pur...
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry testing of human 293T cells. Black: cells alone; Grey: isotype control...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin a...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN3025707) HeLa cells stained with AF488 labeled Nucleolin antibody. Green: AF488-labeled Ab. Bl...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Western blot of A431 cell lysate using Nucleolin antibody.
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 7)
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 8)
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.
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