Mitochondrial Marker antibody

Details for Product No. ABIN6297763
Antigen
Reactivity
Human
124
21
21
21
Host
Mouse
121
3
Clonality
Monoclonal
Conjugate
Un-conjugated
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
117
84
75
49
27
21
21
10
9
6
Options
Purpose Mouse anti-Human Mitochondria Marker Antibody [Sodium Azide Free]
Immunogen The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
Specificity Mitochondria in cytoplasm
Purification The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
Antigen
Alternative Name Mitochondria Marker
Background

Target Description: MAb MTC754 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Application Notes FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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