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Mitochondrial Marker antibody

Reactivity: Human IF, WB, FACS, IHC Host: Mouse Monoclonal unconjugated
Catalog No. ABIN6297763
  • Target
    Mitochondrial Marker
    Reactivity
    • 30
    • 9
    • 9
    Human
    Host
    • 28
    • 2
    Mouse
    Clonality
    • 30
    Monoclonal
    Conjugate
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    Un-conjugated
    Application
    • 28
    • 26
    • 20
    • 11
    • 11
    • 9
    • 9
    • 2
    • 2
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
    Purpose
    Mouse anti-Human Mitochondria Marker Antibody [Sodium Azide Free]
    Specificity
    Mitochondria in cytoplasm
    Purification
    The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
    Immunogen
    The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
  • Application Notes
    FACS: 0.5-1 μg/million cells
    IF: 0.5-1 μg/mL
    WB: 0.5-1 μg/mL
    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    Restrictions
    For Research Use only
  • Buffer
    In 1X PBS ( pH 7.4), BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • Target
    Mitochondrial Marker
    Alternative Name
    Mitochondria Marker
    Background

    Target Description: MAb MTC754 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

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