ATP1A2 antibody (AA 451-479)

Catalog No. ABIN652011

Product Details anti-ATP1A2 Antibody

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Binding Specificity: AA 451-479

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.

Clone: RB19033

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Independent Validation (IF)

Validation #100013 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100013

Date: 05/11/2016

Antigen: ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2)

Lot Number: SH09708BF

Method validated: Immunofluorescence

Positive Control: Wild-type mouse adult brain cryo-sections

Negative Control: Rabbit IgG

Notes: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validation Images

IF validation of ATP1A2 antibody ABIN652011 on mouse brain sections. A. ABIN652011 diluted 1:10 (red), DAPI counterstaining (blue). B. Dilution factor 1:10. C. Dilution factor 1:50. D. Dilution factor 1:200.

Full Methods

Primary Antibody: ABIN652011

Secondary Antibody: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN652011 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Experimental Notes: ABIN652011 worked at 1:10, did not work well in 1:50-1:200.

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATP1A2 antibody (ABIN652011)

Du, Kiyoshi, Wang, Wang, Ma, Alford, Zhong, Wan, Chen, Lloyd, Bryan, Zhou: "Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ." in: Frontiers in cellular neuroscience, Vol. 10, pp. 13, (2016) (PubMed).

Wang, Kiyoshi, Du, Ma, Alford, Chen, Zhou: "mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes." in: Molecular neurobiology, (2015) (PubMed).

Target Details for ATP1A2

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Alternative Name: ATP1A2 (ATP1A2 Products)

Synonyms: C11orf29 antibody, TIF1D antibody, TIF1DELTA antibody, AW060654 antibody, Atpa-3 antibody, mKIAA0778 antibody, RATATPA2 antibody, FHM2 antibody, MHP2 antibody, atp1a2 antibody, atp[a]2 antibody, atpa2 antibody, cb702 antibody, fa10d04 antibody, fa10d05 antibody, wu:fa10d05 antibody, ATP2A antibody, atp1a2.L antibody, fhm2 antibody, mhp2 antibody, ATP1A2 antibody, ATP1A4 antibody, tripartite motif containing 66 antibody, ATPase, Na+/K+ transporting, alpha 2 polypeptide antibody, ATPase Na+/K+ transporting subunit alpha 2 antibody, ATPase, Na+/K+ transporting, alpha 2a polypeptide antibody, sodium/potassium-transporting ATPase subunit alpha-2 antibody, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog antibody, TRIM66 antibody, Atp1a2 antibody, ATP1A2 antibody, atp1a2a antibody, atp1a2.S antibody, LOC488636 antibody

Molecular Weight: 112265

Gene ID: 477

NCBI Accession: NP_000693

UniProt: P50993

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process